Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2008-05-12
2011-12-27
Johannsen, Diana (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S287200, C536S024310
Reexamination Certificate
active
08084203
ABSTRACT:
The invention relates to methods and reagents for the determination of telomere length in tissue sections by the single cell telomeric mapping technique based on a fluorescent in situ hybridization step using a telomere-specific probe and an interpolation step using a standard curve correlating fluorescent intensity and telomere length obtained from a collection of cell lines of known telomere length. The invention further relates to methods for the identification of stem cell niches within tissues and for the identification of compounds capable of triggering stem cell mobilization using the telomere length as criteria for the identification of stem cells and which rely on the single cell telomeric mapping technique of the invention.
REFERENCES:
patent: 6514693 (2003-02-01), Lansdorp
patent: 9641016 (1996-12-01), None
patent: 2004/035597 (2004-04-01), None
patent: 2008081451 (2008-07-01), None
Canela, A. et al. Proceedings of the National Academy of Sciences USA 104(13):5300-5305 (Mar. 27, 2007; published online Mar. 16, 2007).
Baerlocher, G.M. et al. Nature Protocols 1(5):2365-2376 (published online Dec. 21, 2006).
Andres Canela Rodriguez, Estudio de las nuevas funciones de la telomerasa en la tumorigenesis, Thesis Doctoral 2007, pp. 1-257 (cited in 8 parts), publically available Jan. 2008.
Ignacio Flores, et al., The longest telomeres: a general signature of adult stem cell compartments, GENES & DEVELEPMENT, pp. 654-667, vol. 22, Mar. 1, 2008 (online Feb. 18, 2008).
Steven S.S. Poon, et al.,Telonnere Length Measurements Using Digital Fluorescence Microscopy, Cytometry, 1999 pp. 267-278, vol. 36.
Sabita N. Saldanha, et al., Assesment of telomere length and factors that contribute to its stability, Eur. J. Biochem, 2003, pp. 389-403, vol. 270.
Irene Siegl-Cachedenier, et al., Telomerase reverses epidermal hair follicle stem cell defects and loss of long-term survival associated with critically short telomeres, The Journal of Cell Biology, Oct. 2, 2007, pp. 277-290, vol. 179, No. 2.
Ignacio Flores, et al., Telomerase regulation and stem cell behaviour, Current Opinion in Cell Biology, 2006, pp. 254-260, vol. 18
English translation of Conclusion from Andes Canaela Rodriguez Doctoral Thesis, 2007, (3 pages).
Canela, A., et al., “Constitutive expression of tert in thymocytes leads to increased incidence and dissemination of T-cell lymphoma in Lck-Tert mice,” Mol. Cell Biol. May 2004; 24(10):4275-93.
Canela, A., et al., “High-throughput telomere length quantification by FISH and its application to human population studies,” Proc. Natl. Acad. Sci. USA Mar. 27, 2007; 104(13):5300-5.
Flores, I., “The longest telomeres: a general signature of adult stem cell compartments”. 2007 Sometido, 43 page manuscript (vol./No./pp. N/A).
Blasco Marhuenda Maria Antonia
Canela Rodriguez Andrés
Flores Hernández Ignacio
Fuierer Tristan A.
Fundacion Centro Nacional de Investigaciones Oncologicas Carlos
Gerschutz Andrew D.
Johannsen Diana
Moore & Van Allen PLLC
LandOfFree
Methods for the determination of telomere length in a... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for the determination of telomere length in a..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for the determination of telomere length in a... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4308976