Chemistry: analytical and immunological testing – Nitrogen containing – Oxides of nitrogen
Patent
1996-11-08
1999-03-23
Green, Lora M.
Chemistry: analytical and immunological testing
Nitrogen containing
Oxides of nitrogen
436 68, 436118, 436165, 436167, 436168, 422 681, G01N 3350, G01N 2100
Patent
active
058858423
ABSTRACT:
Non-invasive methods have been developed for the measurement of NO levels in a variety of fluid media, e.g., body fluids. The present invention embraces the use of a semi-permeable vessel wherein said vessel contains an NO reacting substance to trap NO diffusing thereinto, and a simple physical or chemical detection method to measure the levels of the end products. Since NO is a neutral gas molecule, it is capable of diffusing freely across a wide range of biocompatible polymer membranes which exhibit a high permeability to NO and other neutral gas molecules, such as O.sub.2 and CO.sub.2, but which are not permeable to charged molecules, such as NO.sub.3.sup.- or NO.sub.2.sup.-. The latter two compounds are ubiquitously present in body fluids and often interfere with the measurement of NO levels. The permeability of selected membranes to NO, but not to NO.sub.3.sup.- or NO.sub.2.sup.-, makes it possible for the bags employed in the practice of the invention to selectively collect NO, even in the presence of potentially competing species. The simple, easy and non-invasive methods of the invention will find a variety of uses, e.g., for diagnosis and monitoring of NO overproduction (and underproduction) that has been associated with many inflammatory and infectious diseases.
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Green Lora M.
Medinox Inc.
Reiter Stephen E.
Ricigliano Joseph W.
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