Methods for the culture of human embryonic stem cells on...

Details Methods for the culture of human embryonic stem cells on... Methods for the culture of human embryonic stem cells on...

2002-08-06

2008-10-07

Crouch, Deborah (Department: 1632)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of co-culturing cells

C435S366000, C435S377000

Reexamination Certificate

active

07432104

ABSTRACT:
Methods and cell culture medium for the generation of human pluripotent embryonic stem cells are disclosed. Human embryonic stem cells are cultured with human granulosa feeder cells, muscle cells, Fallopian ductal epithelial cells, bone marrow stromal cells, and skin fibroblasts and the embryonic stem cells maintain their pluripotent phenotype. The human pluripotent embryonic stem cells can be cultured without feeder cells, and in the presence of supplemental growth factors. The human pluripotent embryonic stem cells can be alternatively cultured with conditioned medium obtained from a cell culture capable of maintaining human embryonic stem cells in a pluripotent state, wherein the cell culture is a human granulosa cell culture.

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Eiges et al. A Molecular View on Pluriopotent Stem Cells, FEBS Letters. 2002, vol. 529, pp. 135-141.
ATCC Catalog, 1994, pp. 171 and 236.

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