Multicellular living organisms and unmodified parts thereof and – Method of using a transgenic nonhuman animal in an in vivo...
Patent
1997-01-21
1999-10-19
Crouch, Deborah
Multicellular living organisms and unmodified parts thereof and
Method of using a transgenic nonhuman animal in an in vivo...
800 14, 424 92, 435 30, C12N 500, C12N 1500, A61K 4900
Patent
active
059692103
ABSTRACT:
One embodiment of the present invention provides a method of testing for compounds which may induce STF-1 transcription. Pancreatic islet cells which express the STF-1/lacz fusion gene are isolated and the effect of various compounds on STF-1 expression is measured by adding the compound of interest to STF-1/lacZ expressing cells. LacZ activity in control and treated cells is then quantitated by a colorimetric assay. Using this method, a large number of compounds can be screened and STF-1 inducing compounds can be identified readily. Another embodiment of the present invention provides a method of using the STF-1 promoter to mark insulin producing pancreatic islet cells in vivo. In this regard, the green fluorescent protein (GFP) served as an indicator. The introduction of the STF-1 green fluorescent protein transgene into pigs allows for the efficient and rapid recovery of insulin producing cells from the pancreas.
Montminy Marc R.
Sharma Seema
Adler Benjamin Aaron
Crouch Deborah
Research Development Foundation
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