Methods for testing oxidative stress

Details Methods for testing oxidative stress Methods for testing oxidative stress

1999-02-19

2000-12-26

Alexander, Lyle A.

Chemistry: analytical and immunological testing

Oxygen containing

Carbonyl, ether, aldehyde or ketone containing

436166, 436130, 436808, G01N 2178, G01N 33493

Patent

active

061657972

ABSTRACT:
Urine is tested for presence of malondialdehyde by mixing it with a reagent consisting of about 90-110 parts 20% acetic acid, about 13.5-16.5 parts Ingredient A, and about 4.5-5.5 parts Ingredient B, wherein Ingredient A is comprised of sodium metabisulfite, phosphoric acid and deionized water in the proportions of about 18-22 grams sodium metabisulfite, 9-11 ml of concentrated phosphoric acid, and about 450-550 ml deionized water, and Ingredient B is comprised of basic fuchsin and Ingredient A in the proportion of about 0.45-0.55 grams basic fuchsin to about 90-110 ml Ingredient A. A color change is observed when malondialdehyde is present in the urine in a concentration greater than about 2 ppm.

REFERENCES:
patent: 4753891 (1988-06-01), Thompson et al.
patent: 5950634 (1999-09-01), Ochi et al.
patent: 5985665 (1999-11-01), Crawford et al.

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