Combinatorial chemistry technology: method – library – apparatus – Method of creating a library – Solid-phase synthesis or particular method of cleavage from...
Reexamination Certificate
2011-07-05
2011-07-05
Lundgren, Jeffrey S. (Department: 1639)
Combinatorial chemistry technology: method, library, apparatus
Method of creating a library
Solid-phase synthesis or particular method of cleavage from...
C506S007000, C506S013000, C506S023000, C506S025000, C506S030000, C435S006120, C435S091200, C536S023100, C530S300000, C530S350000
Reexamination Certificate
active
07972994
ABSTRACT:
The present invention provides methods of synthesizing libraries of molecules comprising a functional moiety which is operatively linked to an encoding oligonucleotide, wherein the encoding oligonucleotide comprises a capping sequence containing degenerate nucleotides. The methods generally include providing a solution comprising initiator compounds comprising an initial functional moiety comprising n building blocks which is operatively linked to an initial oligonucleotide; dividing the solution into reaction vessels; reacting the initiator compounds in each reaction vessel with a building block comprising a complementary reactive group to form a covalent bond; and reacting the initial oligonucleotide in each aliquot with a distinct incoming oligonucleotide in the presence of an enzyme which catalyzes the ligation of the incoming oligonucleotide and the initial oligonucleotide, under conditions suitable for enzymatic ligation of the incoming oligonucleotide and the initial oligonucleotide to form an encoding oligonucleotide.
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Acharya Raksha A.
Arico-Muendel Christopher C.
Benjamin Dennis
Centrella Paulo A.
Clark Matthew
GlaxoSmithKline LLC
Laccotripe Zacharakis Maria
Lundgren Jeffrey S.
McCarter & English LLP
Nagle Deborah L.
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