Methods for studying capillary circulation using fish fry and ta

Details Methods for studying capillary circulation using fish fry and ta Methods for studying capillary circulation using fish fry and ta

1995-07-10

1996-10-15

Campbell, Bruce R.

Drug, bio-affecting and body treating compositions

In vivo diagnosis or in vivo testing

Diagnostic or test agent produces in vivo fluorescence

424 91, 424 92, G01N 3328

Patent

active

055651879

ABSTRACT:
A method is provided for studying capillary circulation utilizing intravital microscopy. The method comprises providing an intact living Salmonid or other Teleost alevin with yolk sac attached in a small water filled plastic bag or tube for immobilizing the fish fry. Thereafter there is injected into the yolk sac, a fluorescent dye complex and tracer FITC-DX (MW 150,000 daltons) and the absorption of the injected material into the systemic circulation of said living Salmonid or Teleost alevin tracked by means of fluorescent microscopy. The method can be used for carrying out toxicological pharmacological, physiological, embryological, oncological, pathological, etc. studies.

REFERENCES:
patent: 3565041 (1971-02-01), Brooks
D E Hinton et al (1989) Mar. Environ. Res. 28:407-410 (Abstract only).
M Romanus et al (1983) Arch Phys Med Rehabil 64:553-555.
VWR Scientific 1989/1990 catalog p. 1498.

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