Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-12-19
1997-02-18
Chambers, Jasemine C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 254, 4351723, C12Q 168, C12N 1510, C07H 2100
Patent
active
056040970
ABSTRACT:
The invention provides a method of tracking, identifying, and/or sorting classes or subpopulations of molecules by the use of oligonucleotide tags. Oligonucleotide tags of the invention each consist of a plurality of subunits 3 to 6 nucleotides in length selected from a minimally cross-hybridizing set. A subunit of a minimally cross-hybridizing set forms a duplex or triplex having two or more mismatches with the complement of any other subunit of the same set. The number of oligonucleotide tags available in a particular embodiment depends on the number of subunits per tag and on the length of the subunit. An important aspect of the invention is the use of the oligonucleotide tags for sorting polynucleotides by specifically hybridizing tags attached to the polynucleotides to their complements on solid phase supports. This embodiment provides a readily automated system for manipulating and sorting polynucleotides, particularly useful in large-scale parallel operations, such as large-scale DNA sequencing, mRNA fingerprinting, and the like, wherein many target polynucleotides or many segments of a single target polynucleotide are sequenced simultaneously.
REFERENCES:
patent: 5302509 (1994-04-01), Cheeseman
patent: 5482836 (1996-01-01), Cantor
Kuijper et al, "Functional cloning vectors for use in directional cDNA cloning using cohesive ends produced with T4 DNA polymerase," Gene, 112: 147-155 (1992).
Aslanidis et al, "Ligation-independent cloning of PCR products (LIC-PCR)," Nucleic Acids Research, 18: 6069-6074 (1990).
Wetmur, "DNA probes: applications of the principles of nucleic and hybridization," Critical Reviews in Biochemistry and Molecular Biology, 26: 227-259 (1991).
Egholm et al, "PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules," Nature, 365: 566-568 (1993).
Gryaznov et al, "Modulation of oligonucleotide duplex and triplex stability via hydrophobic interactions," Nucleic Acids Research, 21: 5909-5915 (1993).
Crick et al, "Codes without commas, " Proc. Natl. Acad. Sci., 43: 416-421 (1957).
Ohlmeyer et al, "Complex synthetic chemical libraries indexed with molecular tags," Proc. Natl. Acad. Sci., 90: 10922-10926 (1993).
Brenner and Lerner, "Encoded combinatorial chemistry," Proc. Natl. Acad. Sci., 89: 5381-5383 (1992).
Maskos and Southern, "Oligonucleotide hybridizations on glass supports: a novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotides synthesized in situ," Nucleic Acids Research, 20: 1679-1684 (1992).
Matthews and Kricka, "Analytical strategies for the use of DNA probes," Anal. Biochem. 169: 1-25 (1988).
Broude et al, "Enhanced DNA sequencing by hybridization," Proc. Natl. Acad. Sci., 91: 3072-3076 (1994).
Nielsen et al, "Synthetic methods for the implementation of encoded combinatorial chemistry," J. Am. Chem. Soc. 115: 9812-9813 (1993).
Needels et al., "Generation and screening of an oligonucleotide-encoded synthetic peptide library," Proc. Natl. Acad. Sci., 90: 10700-10704 (1993).
Chetverin et al, "Oligonucleotide arrays: New concepts and possibilities," Biotechnology, 12: 1093-1099 (1994).
Yang and Youvan, "A prospectus for multispectral-multiplex DNA sequencing," Biotechnology, 7: 576-580 (1989).
Church et al, "Multiplex DNA Sequencing," Science, 240: 185-188 (1988).
Beck et al, "A Strategy for the amplification, purification, and selection of M13 templates for large-scale DNA sequencing," Analytical Biochemistry, 212: 498-505 (1993).
Ji and Smith, "Rapid purification of double-stranded DNA by triple-helix-mediated affinity capture," Anal.Chem., 65: 1323-1328 (1993).
Brown et al., "A new base-stable linker for solid-phase oligonucleotide synthesis," J. Chem. Soc. Commun. 1989: 891-893.
Oliphant et al, "Cloning of random-sequence oligodeoxynucleotides," Gene, 44: 177-183 (1986).
Hunkapiller et al, "Large-scale and automated DNA sequence determination," Science, 254: 59-67 (1991).
Coche et al., "Reducing bias in cDNA sequence representation by molecular selection," Nucleic Acids Research, 22: 4545-4546 (1994).
Chambers Jasemine C.
Macevicz Stephen C.
Priebe Scott D.
Spectragen, Inc.
LandOfFree
Methods for sorting polynucleotides using oligonucleotide tags does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for sorting polynucleotides using oligonucleotide tags, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for sorting polynucleotides using oligonucleotide tags will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1601825