Methods for sorting dimorphic daughter cells

Details Methods for sorting dimorphic daughter cells Methods for sorting dimorphic daughter cells

2007-02-16

2011-12-27

Gabel, Gail R (Department: 1641)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

C435S040510, C435S325000, C435S347000, C435S289100, C436S063000, C436S164000

Reexamination Certificate

active

08084257

ABSTRACT:
This invention relates to methods for distinguishing and sorting cells. In particular it includes methods for distinguishing and sorting post-mitotic and post-meiotic daughter cells into two classes according to differential cellular features. Labeling, tagging, or marking of the cells' chromatin proteins, RNA, or DNA may assist in distinguishing the daughter cells. In some embodiments, two cell classes may be studied and the cells' proteins, glycoproteins, and RNA may be identified and subset. Information from these subsets may then be used to distinguish and sort the two classes of cells from similar tissues according to protein, glycoprotein, and RNA makeup.

REFERENCES:
patent: 6620591 (2003-09-01), Dunlay et al.
patent: 2003/0124505 (2003-07-01), Jain et al.
Kolas et al., Mutant meiotic chromosome core components in mice can cause apparent sexual dimorphic endpoints at prophase or X-Y defective male-specific sterility. Chromosoma, (Jul. 2005) vol. 114, No. 2, pp. 92-102.

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