Methods for simultaneous isolation of biologically active...

Details Methods for simultaneous isolation of biologically active... Methods for simultaneous isolation of biologically active...

2005-07-26

2005-07-26

Whisenant, Ethan (Department: 1634)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S006120, C530S350000, C530S412000

Reexamination Certificate

active

06921817

ABSTRACT:
A rapid isolation method permits the isolation of DNAs, RNAs and transcription factors simultaneously from a wide range of lymphoid and non-lymphoid cells, either from patients or in culture, without the loss of biological activity. A significant advantage of this method is that it entails minimal handling of samples from the patients with various disease states and pathogenic conditions such as AIDS, hepatitis, and other infectious diseases, which require substantial precautions during isolation of DNAs and proteins.

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Caruccio et al., J. of Immunological Methods 230 : 1-10 (Nov. 1999).
Dignam et al., Nucleic Acids Research 11(5) : 1475-1489 (1983).

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