Methods for sequencing polymers using mass spectrometry

Details Methods for sequencing polymers using mass spectrometry Methods for sequencing polymers using mass spectrometry

1997-04-18

1998-10-13

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435183, 435195, 435199, 435200, 435 18, 250282, 250288, 935 77, C12Q 168, C12Q 134, C12N 914, C12N 924

Patent

active

058210636

ABSTRACT:
The methods and apparatus disclosed herein are useful for sequencing polymers using mass spectrometry. The methods involve differing ratios of hydrolyzing agent to polymer disposed upon a reaction surface adapted for use with a mass spectrometer. The methods further involve integrating data obtained from mass spectrometry analysis of a plurality of series of hydrolyzed polymer fragments, and optionally provide statistical interpretation paradigms and computer software therefor. The apparatus involves a mass spectrometer sample holder, having hydrolyzing agent disposed thereon, which is useful for adapting any mass spectrometer for polymer sequencing.

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