Methods for separating casein from soluble proteins in a...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

Reexamination Certificate

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C530S412000, C530S351000, C530S360000, C530S361000, C530S381000, C530S380000, C530S832000, C210S702000, C210S705000, C210S723000, C210S724000, C426S034000, C426S041000, C426S055000

Reexamination Certificate

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07943739

ABSTRACT:
A method for precipitating casein from a suspension comprising milk is disclosed. The method includes the following steps: adding a phosphate solution to a suspension; mixing the phosphate solution with the suspension to form a mixture having a phosphate concentration greater or equal to 40 mM; freezing the mixture having a phosphate concentration greater or equal to 40 mM to obtain a frozen mixture; and thawing the frozen mixture to obtain casein-containing aggregates in the mixture, in which the phosphate solution is buffered at a pH value of no less than 4.4.

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patent: 6355271 (2002-03-01), Bell et al.
patent: 1115745 (2006-05-01), None
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Lonergan D A: “Isolation of casein by ultra filtration and cryo destabilization” Journal of Food Science, vol. 48, No. 6, 1983, pp. 1817-1821.
C. Guo a, et al., “Casein precipitation equilibria in the presence of calcium ions and phosphates” Colloids and Surfaces B: Biointerfaces 29 (2003) 2970/307.
Tulin Morcol, Quing He and Stever J, D, Bell, Model Process for Removal of Caseins from Milk of Transgenic Animals, Blotechnol, Prog. 17: 577-582, 2001.

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