Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2007-08-07
2007-08-07
Vogel, Nancy (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S252320
Reexamination Certificate
active
10673860
ABSTRACT:
The object of the present invention is to provide a method of producing a heterologous protein by making a coryneform bacterium to produce and efficiently extracellularly secrete (secreto-production) an industrially useful heterologous protein. According to the present invention, a genetic construct is used where a gene sequence encoding an intended protein which is ligated to the downstream of a sequence encoding the signal peptide derived from a coryneform bacterium, the gene construct is introduced into a mutant coryneform bacterium which has a capacity of secreting the heterologous protein at least 2-fold higher than the wild typeCorynebacterium glutamicumATCC 13869, the mutant coryneform bacterium is cultured and the extracellularly released heterologous protein is recovered.
REFERENCES:
patent: 4822738 (1989-04-01), Miwa et al.
patent: 4965197 (1990-10-01), Liebl et al.
patent: 5547864 (1996-08-01), Kawasaki et al.
patent: 5643790 (1997-07-01), Morinaga et al.
patent: 6027920 (2000-02-01), Joliff et al.
patent: 6090607 (2000-07-01), Van Den Broek et al.
patent: 10108675 (1998-04-01), None
patent: 11169182 (1999-06-01), None
patent: WO88/09821 (1988-12-01), None
patent: WO93/03158 (1993-02-01), None
patent: WO01/23591 (2001-04-01), None
Helen Billman-Jacobe et al., Expression and Secretion of Heterologous Proteases byCorynebacterium glutamicum, Journal of Bacteriology, Mar. 1992, p. 1854-1861, vol. 174, No. 6.
Wolfgang Liebl et al., Expression, Secretion, and Processing of Staphylococcal Nuclease byCorynebacterium glutamicum, Applied and Environmental Microbiology, Apr. 1995, p. 1610-1613, vol. 61, No. 4.
K. Salim et al., Heterologous Expression of the Mycobacterium tuberculosis Gene Encoding Antigen 85A inCorynebacterium glutamicum, Applied and Environmental Microbiology, Nov. 1997, p. 4392-4400, vol. 63, No. 11.
Kinya Washizu et al., Molecular Cloning of the Gene for Microbial Transglutaminase fromStreptoverticilliumand Its Expression inStreptomyces lividans, Biosci. Biotech. Biochem., 58(1), 82-87, 1994.
Shino Takihana et al., Chemical Synthesis of the Gene for Microbial Transglutaminase fromStreptoverticilliumand Its Expression inEscherichia coli, Biotech. Biochem., 58(1), 88-92, 1994.
R Duran et al., Purification, characterization, and gene cloning of transglutaminase fromStreptoverticillium.
Int'l Search Report, Jun. 25, 2002, Japan Patent Office.
Int'l Preliminary Examination Report, Nov. 26, 2002, Japan Patent Office.
Ralf Pasternack et al., Bacterial pro-transglutaminase fromStreptoverticillium mobaraensePurification, characterization and sequence of the zymogen, Eur. J. BIochem. 257, 570-576 (1998).
J. L. Peyret et al, Characterization of the cspB gene encoding PS2, an ordered surface-layer protein inCorynebacterium glutamicum, Molecular Microbiology (1993) 9(1), 97-109.
U.S. Appl. No. 10/149,450, filed Jun. 27, 2002, Nakanishi et al.
Date Masayo
Heima Haruo
Kikuchi Yoshimi
Matsui Hiroshi
Umezawa Yukiko
Ajinomoto Co. Inc.
Cermak Shelly Guest
Cermak & Kenealy LLP
Vogel Nancy
LandOfFree
Methods for secretory production of proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for secretory production of proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for secretory production of proteins will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3890632