Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-12-25
2007-12-25
Sisson, Badley L. (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S183000, C435S287200, C436S094000, C536S023100, C536S024310, C536S024330
Reexamination Certificate
active
10001486
ABSTRACT:
The present invention provides a method of screening for a compound that binds to a selected nucleic acid comprising contacting compound fluorescently labeled by a fluorescent protein with a cell having a plurality of copies of the nucleic acid in an array such that the nucleic acid can be directly detected when bound by fluorescently labeled compound; and directly detecting the location of fluorescence within the cell, fluorescence aggregated at the site of the nucleic acid array indicating a compound that binds to the selected nucleic acid. In particular compounds such a transcription factors can be screened. Reagents for such method are provided including a mammalian cell having a plurality of steroid receptor response elements in an array such that the response element can be directly detected when bound by fluorescently labeled steroid receptor and a chimeric protein comprising a fluorescent protein fused to a steroid receptor.
REFERENCES:
patent: WO/9507463 (1995-03-01), None
patent: WO/9521191 (1995-08-01), None
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Hager Gordon
Htun Han
Htun Han
Mandel & Adriano
Sisson Badley L.
The United States of America as represented by the Secretary of
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