Methods for screening GABA-modulatory compounds for...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007100, C435S069100, C435S252300, C435S320100, C436S501000, C536S063000

Reexamination Certificate

active

07060451

ABSTRACT:
Methods are provided that are useful in assaying compounds for cognitive enhancing properties, anxiolytic properties, hypnotic properties, or antidepressant properties. These methods involve determining the in vitro efficacy and EC50of the compounds at defined series of cloned GABAAsubtype receptors composed of specific variants of α, β, and γ subunits in order to develop and an activity profile for each compound. Optionally, the binding affinities of the compounds at GABAAreceptors are also determined. As an additional step the in vivo effects of the compounds may be tested in animal models.

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