Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2005-11-01
2005-11-01
Landsman, Robert S. (Department: 1647)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007200, C435S006120
Reexamination Certificate
active
06960440
ABSTRACT:
This invention provides an isolated nucleic molecule encoding a polypeptide capable of binding a p75NTRreceptor, and a purified version of said polypeptide capable of binding a p75NTRreceptor. This invention provides a method of producing a purified polypeptide capable of binding a p75NTRreceptor. This invention provides an antisense oligonucleotide having a nucleic acid sequence capable of specifically hybridizing to an mRNA molecule encoding the above described polypeptide. This invention provides a method producing a polypeptide capable of binding p75NTrecptor into a suitable vector. This invention provides a method of inducing apoptosis, a method of determining physiological effects, a method for identifying an apoptosis inducing or inhibiting compound, a method for screening cDNA libraries of said polypeptide, a method to induce caspase-2 and caspase-3 activity to cleave poly (ADP-ribose) polymerase and fragment nuclear DNA in a cell, a method to inhibit NF-κB activation in a cell, a method to detect a neurodegenerative disease, a method of producing the isolated human HGR74 protein into a suitable vector, a pharmaceutical composition comprising a purified polypeptide capable of binding a p75NTRreceptor and a pharmaceutically acceptable carrier and a method of identifying a compound which is an apoptosis inhibitor.
REFERENCES:
Pharmacia Catalog, p. 130, 1994.
Sambrook, Molecular Cloning Laboratory Manual, 1989, pp. 17.2, 17.25, 17.34, 17.36.
Hillier et al. Genbank Accession No. N34237, Jan. 1996.
NCI-CGAP. Genbank Accession No. AA576958, Sep. 1997.
Rapp et al. Genbank Accession No. M38188, Mar. 1995.
Lee et al. Genbank Accession No. AI227867, Jan. 1999.
Brown et al. Genbank Accession No. AF097438, Apr. 1999.
Faria et al. Genbank Accession No. AF051347, Oct. 1998.
Brown et al. Genbank Accession No. AF097440, Apr. 1999.
Marra et al. Genbank Accession No. AI118980, Sep. 1998.
Marra et al. Genbank Accession No. W46041, May 1996.
Brown A.L. and Kay G.F., Bex 1, A Gene With Increased Expression In Parthenogenetic Embryos, Is A Member Of A Novel Gene Family On The Mouse X Chromosome,Human Molecular Genetics(1999) vol. 8, No. 4, p. 611-619.
Ye X., Mehlen P., Rabizadeh S., VanArsdale T., Zhang H., Shin H., Wang J.J.L., Leo E., Zapata J., Hauser C. A., Reed J.C. and Bredesen D.E., TRAF Family Proteins Interact With The Common Neurotrophin Receptor And Modulate Apoptosis Induction,The Journal of Biological Chemistry(1999) vol. 274, No. 42, p. 30202-30208.
Merja S.H. et al., Nerve Growth Factor Signalling Through p75 Induces Apoptosis In Schwann Cells Via A Bcl-2-Independent Pathway,The Journal of Neuroscience(1999) vol. 19, p. 4828-4838.
Khursigara G., Orlinick J.R. and Chao M.V., Association Of The p75 Neurotrophin Receptor With TRAF6,The Journal of Biological Chemistry(1999) vol. 274, No. 5, p. 2597-2600.
Strausberg R., Nerve Growth Factor Receptor Associated Protein, Database NCBI Online!, May 8, 2002, Database Accession No. BCX027815 XP002234199.
Baeuerle, P.A. & Henkel, T., (1994) Function and Activation of NF-kappa B in the Immune System.Annu. Rev. Immunol. 12, 142-179 (Exhibit 1).
Barrett, G. L. & Bartlett, P. F., (1994) The p75 Nerve Growth Factor Receptor Mediates Survival or Death Depending on the Stage of Sensory Neuron Development.Proc. Natl. Acad. Sci. USA 91, 6501-6505 (Exhibit 2).
Breeden, L. & Nasmyth, K., (1985) Regulation of the Yeast HO Gene.Cold Spring Harbor Symp. Quant. Biol.vol. L, 643, 650 (Exhibit 3).
Bunone, G., Mariotti, A., Compagni, A., Morandi, E. & Della Valle, G., (1997) Induction of Apoptosis by p75 Neurotrophin Receptor in Human Neuroblastoma Cells.Oncogene 14, 1463-1470 (Exhibit 4).
Carter, B. D., Kaltschmidt, B., Offenhauser, N., Bohn-Matthaei, R., Baeuerle, P. A. & Barde, Y. A., (1996) Selective Activation of NF-kappa B by Nerve Growth Factor Through the Neurotrophin Receptor p75.Science 272, 542-545 (Exhibit 5).
Casaccia-Bonnefil, P., Carter, B. D., Dobrowsky, R. T. & Chao, M. V., (1996) Death of Oligodendrocytes Mediated by the Interaction of Nerve Growth Factor with its Receptor p75.Nature 383, 716-719 (Exhibit 6).
Chao, M. V. & Hempstead, B. L., (1995) p75 and Trk: a Two-receptor System.Trends Neuroscience 18, 321-326 (Exhibit 7).
Ciechanover, A., (1998) The Ubiquitin-proteasome Pathway: on Protein Death and Cell Life.The EMBO Journal 17(24), 7151-7160 (Exhibit 8).
Datta, R., Banach, D., Kojima, H., Talanian, R. V., Alnemri, E. S., Wong, W. & Kufe, D.W., (1996) Activation of the CPP32 Protease in Apoptosis Induced by 1-beta-D-Arabinofuranosylcytosine and Other DNA-Damaging Agents.Blood 88, 1936-1943 (Exhibit 9).
Feinstein, E., Kimchi, A., Wallach, D., Boldin, M. & Varfolomeev, E., (1995) The Death Domain: A Module Shared by Proteins with Diverse Cellular Functions.Trends Biochem. Sci. 20, 342-344 (Exhibit 10).
Fernandes-Alnemri, T., Litwack, G. & Alnemri, E. S., (1994) CPP32, a Novel Human Apoptotic Protein with Homology to Caenorhabditis Elegans Cell Death Protein Ced-3 and Mammalian Interleukin-1 Beta-converting Enzyme.J. Biol. Chem. 269(49), 30761-30764 (Exhibit 11).
Fields, S. & Song, O., (1989) A Novel Genetic System to Detect Protein-protein Interactions.Nature 340, 245-246 (Exhibit 12).
Frade, J. M., Rodriguez-Tebar, A. & Barde, Y.A., (1996) Induction of Cell Death by Endogenous Nerve Growth Factor Through its p75 Receptor.Nature 383, 166-168 (Exhibit 13).
Gavrieli, Y., Sherman, Y. & Ben-Sasson, S. A., (1992) Identification of Programmed Cell Death in situ via Specific Labeling of Nuclear DNA Fragmentation.J. Cell Biol. 119(3), 493-501 (Exhibit 14).
Gietz, D., Jean, A. S., Woods, R. A. & Schiestl, R. H., (1992) Improved Method for High Efficiency Transformation of Intact Yeast Cells.Nucl. Acids Res. 20(6), 1425 (Exhibit 15).
Ito, H., Fukuda, Y., Murata, K. & Kimura, A., (1983) Transformation of Intact Yeast Cells Treated with Alkali Cations.J. Bacteriol. 153(1), 163-168 (Exhibit 16).
Johnson, D., Lanathan, A., Buck, C. R., Sehgal, A., Morgan, C., Mercer, E., Bothwell, M. & Chao, M.., (1986) Expression and Structure of the Human NGF Receptor.Cell 47, 545-554 (Exhibit 17).
Kaplan, D. R. & Miller, F. D., (1997) Signal Transduction by the Neurotrophin Receptors.Curr. Opin. Cell Biol. 9, 213-221 (Exhibit 18).
Khursigara, G., Orlinick, J. R. & Chao, M. V., (1999) Association of the p75 Neurotrophin Receptor with TRAF6J. Biol. Chem. 274(5), 2597-2600 (Exhibit 19).
Lezoualc'h, F., Sagara, Y., Holsboer, F. & Behl, C., (1998) High Constitutive Nf-kappa B Activity Mediates Resistance to Oxidative Stress in Neuronal Cells.J. Neurosci. 18(9), 3224-3232 (Exhibit 20).
Liepinsh, E., Ilag, L. L., Otting, G. & Ibanez, C.F., (1997) NMR Structure of the Death Domain of the p75 Neurotrophin Receptor.The EMBO Journal 16(16), 4999-5005 (Exhibit 21).
Murphy, T., Cleveland, M., Kulesza, P., Magram, J., Murphy, K., (1995) Regulation of Interleukin 12 p40 Expression through an NF-κB Half-Site.Molecular and Cellular Biology 15(10), 5258-5267 (Exhibit 22).
Nakielny, S. & Dreyfuss, G., (1997) Nuclear Export of Proteins and RNAs.Curr. Opin. Cell Biol. 9, 420-429 (Exhibit 23).
Pan, J.,McEver, R. P., (1995) Regulation of the Human P-selection Promoter by Bcl-3 and Specific Homodimeric Members of the NF-κB/Rel Family.J. Biol. Chem. 270(39), 23077-23083 (Exhibit 24).
Pietravalle, F., Lecoanet-Henchoz, S., Blasey, H., Aubry, J. P., Elson, G., Edgerton, M. D., Bonnefoy, J. Y. & Gauchat, J. F., (1996) Human Native Soluble CD40L is a Biologically Active Trimer, Processed Inside Microsomes.J. Biol. Chem. 271(11), 5965-5967 (Exhibit 25).
Rabizadeh, S., Oh, J., Zhong, L., Yang, J., Bitler, C. M., Butcher, L. L. & Bredesen, D. E., (1993) Induction of Apoptosis by the Low-affinity NGF Receptor.Science 261, 345-348 (Exhibit 26).
Rapp, G., Freudenstein, J., Klaudiny, J., Mucha, J., Wempe, F., Zimmer, M. & Scheit, K. H., (1990) Characterization of Three Abundant mRNAs from Human Ovarian Granulosa Cells.DNA and Cell Biol. 9(7), 479-485 (Exhibit 2
Cooper & Dunham LLP
Landsman Robert S.
The Trustes of Columbia University in the City of New York
White, Esq. John P.
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