Methods for removing heavy metals from aqueous solutions and for

Details Methods for removing heavy metals from aqueous solutions and for Methods for removing heavy metals from aqueous solutions and for

1995-06-05

1999-06-01

Hutzell, Paula K.

Chemistry: analytical and immunological testing

Involving antibody fragments

436538, 435 71, 435345, 5303881, 5303889, 530413, 424 7834, 424609, G01N 3353

Patent

active

059087900

ABSTRACT:
The invention relates to monoclonal antibodies (mAB) which bind to water-soluble complexones such as ethylenediamine tetraacetate (EDTA) or diethylenetriamine pentaacetate (DTPA) with high specificity and avidity and retain their high avidity and specificity for these complexones after complexing of EDTA or DTPA with metal ions. These mAB can therefore be used, e.g. coupled on filters or other supports, for removing toxic heavy metals which are complexed with EDTA or DTPA. Furthermore, these mAB are suitable as components of immunoassays (RIA, ELISA etc.) for the quantitative determination of EDTA or DTPA in aqueous solutions.

REFERENCES:
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M.W. Brechbiel, "Synthesis of 1-(p-Isothiocyanatobenzyl) Derivatives of DTPA and EDA. Antibody Labeling and Tumor-Imaging Studies," Inorganic Chemistry, 25(16):2272-2781 (1986).
M. Shulman et al., "A Better Cell Line for Making Hybridomas Secreting Specific Antibodies," Nature, 276:269-270 (1978).
R. Orlandi et al., "Cloning Immunoglobulin Variable Domains for Expression by the Polymerase Chain Reaction," Proc. Nat'l. Acad. Sci., USA, 86:3833-3837 (May 1989).
D.T. Reardan et al., "Antibodies against metal chelates," Nature, 316:265-267 (1985).
Goding, Monoclonal Antibodies, pp. 76-88 (1986).
R.J. Robins, "The Measurement of Low Molecular-Weight, Non-Immunogenic Compounds by Immunoassay," Immunology in Plant Science, pp. 86-141 (1986).
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