Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2003-04-11
2010-11-09
Benzion, Gary (Department: 1637)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S091210
Reexamination Certificate
active
07829312
ABSTRACT:
This invention relates generally to a method for quantifying the number of occurrences of a specific nucleic acid sequence within a nucleic acid sample in order to circumvent the shortcomings of the methods currently available and to provide reliable quantification of a specific nucleic acid sequence within a nucleic acid sample. The present invention provides a method of assessing an amount of a known target nucleic acid sequence in a sample comprising co-amplifying said target nucleic acid sequence and a known amount of a known control nucleic acid sequence to produce respective target and control amplicons, wherein said control nucleic acid sequence is different than said target nucleic acid sequence; and determining relative amounts of said respective amplicons by determining relative quantities of a primer extension reaction using each of said respective amplicons as a template.
REFERENCES:
patent: 5705365 (1998-01-01), Ryder et al.
patent: 5840487 (1998-11-01), Nadeau et al.
patent: 5858658 (1999-01-01), Haemmerle et al.
patent: 6210891 (2001-04-01), Nyren et al.
patent: 2003/0054386 (2003-03-01), Antonarakis et al.
patent: 2005/0089862 (2005-04-01), Therianos et al.
patent: 00/63437 (2000-10-01), None
patent: WO 00/63437 (2000-10-01), None
patent: 01/42496 (2001-06-01), None
patent: 02/20837 (2002-03-01), None
patent: WO 02/20837 (2002-03-01), None
Pourmand et al. Multiplex Pyrosequencing. Nucleic acids research, vol. 30, No. 7, pp. e31/1-5, 2002.
Ronaghi et al. Journal of Chromoatography B, vol. 782, pp. 62-72, 2002.
Alderborn, Anders, et al., “Determination of Single-Nucleotide Polymorphisms by Real-Time Pyrophosphate DNA Sequencing,” Genome Research, 10:1249-1258 (2000).
Bernard, Philip S., et al., “Real-Time PCR Technology for Cancer Diagnostics,” Clinical Chemistry, 48(8):1178-1185 (2002).
Pielberg, Gerli, et al., “Unexpectedly High Allelic Diversity at the KIT Locus Causing Dominant White Color in the Domestic Pig,” Genetics, 160:305-311 (2002).
Pielberg, Gerli, et al., “A Sensitive Method for Detecting Variation in Copy Numbers of Duplicated Genes,” Genome Research, 13:2171-2177 (2003).
Qiu, Ping, et al., “Quantification of Single Nucleotide Polymorphisms by Automated DNA Sequencing,” Biochemical and Biophysical Research Communications, 309:331-338 (2003).
Reis, Patricia P., et al., “Quantitative Real-Time PCR Identifies a Critical Region of Deletion on 22q13 Related to Prognosis in Oral Cancer,” Oncogene, 21:6480-6487 (2002).
Ronaghi, Mostafa, “Pyrosequencing Sheds Light on DNA Sequencing,” Genome Research, 11:3-11 (2001).
Harris et al., Science, 320(5872):106-109 (2008). “Single Molecule DNA Sequencing of a Viral Genome.”
Hubert Nathalie
Laforest Martin
Landry Benoît S.
Benzion Gary
DNA Landmarks, Inc.
Nixon & Peabody LLP
Wilder Cynthia B
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