Methods for reducing the formation of by-products in the...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide

Reexamination Certificate

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C435S071200

Reexamination Certificate

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07547529

ABSTRACT:
A method for reducing the formation of a byproduct polypeptide containing an O-acetylserine residue in place of a serine residue by adding at least one of histidine, methionine or glycine to the medium in a method for producing a polypeptide containing a serine residue by culturing transformed cells, and a method for producing a polypeptide containing a serine residue by culturing transformed cells, characterized by reducing the formation of a byproduct polypeptide containing an O-acetylserine residue in place of a serine residue by adding at least one of histidine, methionine or glycine to the medium.

REFERENCES:
patent: 4987070 (1991-01-01), Magota et al.
patent: 5169772 (1992-12-01), Zimmerman et al.
patent: 5599690 (1997-02-01), Fenton et al.
patent: 5622845 (1997-04-01), Brunner et al.
patent: 5670340 (1997-09-01), Yabuta et al.
patent: 5698418 (1997-12-01), Brunner et al.
patent: 2003/0170811 (2003-09-01), Ueda et al.
patent: 0164273 (1985-12-01), None
patent: 0683233 (1995-11-01), None
patent: 2180539 (1987-03-01), None
patent: 61119189 (1986-06-01), None
patent: 62044198 (1987-02-01), None
patent: 687788 (1989-01-01), None
patent: 2879063 (1991-06-01), None
patent: WO 90/10706 (1990-09-01), None
Power et al., Manual of BBL Products and Laboratory Procedures, six edition, 1988. pp. 1-3 as attached.
Atlas et al., handbook of Microbiological Media, second edition, 1997. pp. 1-2 as attached.
Schreiner et al., “Metabolism of methionine and methionine hydroxy analogue by porcine kidney fibroblasts,” The Journal of Nutrition, Sep. 1987, vol. 117, No. 9, pp. 1541-1549.
Supplementary European Search Report dated Jul. 22, 2004 for European Application No. 01930035.9-1212-JP0103909.
Burnell et al., “Sulphur metabolism inParacoccus denitrificans. Purification, properties and regulation of serine transacetylase, O-acetylserine sulphydrylase and beta-cystathionase.” Biochimica et Biophysica, 1977, vol. 481, pp. 246-265.
Leu et al. “Kinetic mechanism of serine transacetylase fromSalmonella typhimurium.” Biochemistry, 1994, vol. 33, pp. 2667-2671.
European Patent Office Examination dated Dec. 23, 2004 for European Application No. 01930035.9-1212.
Tsai, Larry B. et al., “Control of Misincorporation ofDe NovoSynthesized Noreleucine into Recombinant Interleukin-2 inE. Coli,” Biochemical and Biophysical Communications, 156(2):733-739 (1988).
Bogosian, George, “Biosynthesis and Incorporation into Protein of Norleucine byEscherichia coli,” The Journal of Biological Chemistry, 264(1):531-539 (1989).
Apostol, Izydor, et al., “Incorporation of Norvaline at Leucine Positions in Recombinant Humah Hemoglobin Expressed inEscherichia coli,” The Journal of Biological Chemistry, 272(46):28980-28988 (1997).
Kangawa, Kenji, et al., “Purification and Complete Amino Acid Sequence of α-Human Atrial Natriuretic Polypeptide (α-hANP),”Biochemical and Biophysical Research Communications, 118(1):131-139 (1984).
Masaki, Takeharu: et al., “Studies on a New Proteolytic Enzyme fromAchromobacter LyticusM4971 II. Specificity and Inhibition Studies ofAchromobacterProtease 1,”Biochimica et Biophysica Acta, 660:51-55 (1981).
Neidhardt, Frederick C., et al., “Chemical Composition ofEscherichia coli,” Escherichia coli and Salmonella Cellular and Molecular Biology, vol. I, pp. 13-16, ASM Press, Washington, DC (1996).
Twigg, Andrea J., et al., “Trans-Complementiable Copy-Number Mutants of Plasmid CoIE 1,”Nature, 283:216-218 (1980).
Kredich, Nicholas M., “Biosynthesis of Cysteine,”Escherichia coli and Salmonella Cellular and Molecular Biology, vol. I, pp. 514-527, ASM Press, Washington, DC (1996).

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