Chemistry: electrical and wave energy – Processes and products – Processes of treating materials by wave energy
Patent
1994-07-05
1995-07-04
Niebling, John
Chemistry: electrical and wave energy
Processes and products
Processes of treating materials by wave energy
2041531, 204403, 435817, G01N 2726
Patent
active
054297265
ABSTRACT:
Improved methods and compositions are provided to inhibit interference from formaldehyde in polarographic analytic methods. In the enzymatic oxidation of methanol to hydrogen peroxide and formaldehyde, formaldehyde serves as an interfering agent in systems where the oxygen consumed or the hydrogen peroxide produced is polarographically measured. To inhibit this interference, an amine-containing formaldehyde scavenger is added to the test system. Preferably, the scavenger comprises a polyalkyleneimine such as polyethyleneimine having a molecular weight of between about 200-1,000,000 amu. The invention is particularly well-suited for use in polarographic analytical apparatus wherein laminated membrane type enzyme electrodes are used to perform amperometric measurement with alcohol oxidase enzyme used as the oxidoreductase enzyme. Determination of methanol concentration in liquid samples can be used to assess the amount of L-Asp-L-Phe-methyl ester (aspartame-TM) present in diet soft drink samples that have been hydrolyzed with the enzyme Bovine alpha-chymotrypsin to yield L-Asp-L-phe and methanol.
REFERENCES:
patent: 4855353 (1989-08-01), Kurami et al.
Johnson Jay M.
Spokane Robert B.
Bell Bruce F.
Niebling John
The Yellow Springs Instrument Company, Inc.
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