Methods for reducing inhibition of nucleic acid amplification re

Details Methods for reducing inhibition of nucleic acid amplification re Methods for reducing inhibition of nucleic acid amplification re

1995-09-28

1997-04-15

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 912, 435 6, 435 5, 536 243, 536 2432, 536 2433, C12P 1934, C12Q 168, C12Q 170, C07H 2104

Patent

active

056208693

ABSTRACT:
It has been found that certain glycoproteins, particularly mucins, are inhibitors of nucleic acid amplification reactions and that inhibition of the amplification reaction is associated with partial degradation of the carbohydrate chain. Partial degradation of the carbohydrate of a non-inhibitory glycoprotein renders it inhibitory, and partial degradation of the carbohydrate of a slightly inhibitory glycoprotein makes it more inhibitory. Sample processing prior to amplification may contribute to partial degradation of the carbohydrate chains of the glycoproteins which are present and increase their inhhibitory effect. In contrast, complete removal of the carbohydrate significantly reduces or completely eliminates the inhibitory effect. Methods for reducing or eliminating glycoprotein-associated inhibition of nucleic acid amplification reactions are also disclosed.

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