Methods for reducing enzyme consumption in second generation...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

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C435S014000, C435S209000

Reexamination Certificate

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07972826

ABSTRACT:
Provided are methods of liquefaction, saccharification and fermentation of pre-treated lignocellulosic biomass in production of bioethanol. Addition of PEG or surfactant is effective to enhance cellulase hydrolysis yields at high dry matter (>20%) at surprisingly low concentrations compared with comparable results in low dry matter hydrolysis. This effect is most pronounced at comparatively low cellulase loading <7 FPU (g DM)−1. At high dry matter content, cellulase enzyme consumption can be reduced, without loss of hydrolysis yield, by addition of small quantities of PEG or surfactant.

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