Methods for reducing background binding in antibody preparations

Details Methods for reducing background binding in antibody preparations Methods for reducing background binding in antibody preparations

1995-06-23

1998-06-02

Scheiner, Toni R.

Chemistry: analytical and immunological testing

Involving production or treatment of antibody

436825, 435 72, 435 721, 435 725, 435 4051, 435 4052, 435962, 53038826, 5303891, C01N 3353, C01N 33573, G07K 1600

Patent

active

057598649

ABSTRACT:
In accordance with the present invention, there are provided novel methods and reagents for reducing background binding in antibody preparations having an unwanted affinity for intracellular protein(s). The invention method comprises treating an antibody preparation with permeabilized cells, then separating the antibody preparation from the permeabilized cells. The reagents are useful for reducing the level of background binding in antibody preparations. Also provided are antibody preparations that are substantially free of background binding.

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