Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2004-03-19
2009-08-11
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200
Reexamination Certificate
active
07572577
ABSTRACT:
The invention provides a method for recovery of cDNA from mRNA, comprising reverse transcription (RT) of mRNA using a RT primer which includes a sequence based on the 5′ consensus region of the mRNA which is identical or similar to 5′ consensus region of the mRNA and which includes a sequence capable of specifically hybridising to the 3′ region of the mRNA, followed by polymerase chain reaction (PCR) using a single primer to generate ss cDNA, ds cDNA and amplify the cDNA. Primers for use in methods of the invention and kits for performing methods of the invention are also provided. The methods of the invention can partially or fully automated.
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United Kingdom Patent Office Search Report for Patent Application Serial No. 0306305, dated Aug. 12, 2003, 1 page.
He Mingyue
Jackson Alison M.
Crescendo Biologics Limited
Goodwin & Procter LLP
Horlick Kenneth R.
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