Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-08-02
1998-08-04
Wilson, James O.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 72, 435 721, 435 731, 435 732, 435 791, 435 32, 436518, 436530, 436809, 536 253, 536 2531, 536 2532, 536 254, 536 2541, 356201, 356442, C12Q 168
Patent
active
057891737
ABSTRACT:
The present invention discloses a method for rapid antimicrobial susceptibility testing to screen antibiotics in a few hours instead of days by conventional methods. This method can also be used to identify susceptible antibiotics to treat mycobacterial infection in a few days instead of the usual six to eight weeks. Fast screening of antibiotics is achieved by a short period of specimen incubation in different antibiotics embedded media to create differential bacterial counts. The differences of bacterial counts among antibiotics embedded media are subsequently amplified by DNA amplification methods for detection. Following DNA amplification, rapid quantitation and minimum inhibition concentration (MIC) determinations for a panel of antibiotics are achieved in less than one minute by fluorescence quantitation methods.
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Peck Konan
Wung Shu-Li
Yang Pan-Chyr
Academia Sinica
Wilson James O.
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