Methods for purifying nucleic acids

Details Methods for purifying nucleic acids Methods for purifying nucleic acids

1998-07-23

2010-10-05

Vogel, Nancy (Department: 1636)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S091100

Reexamination Certificate

active

07807822

ABSTRACT:
Methods are provided for producing highly purified compositions of nucleic acids by using a highly streamlined and readily automated process. The methods use static mixers for lysing cells and precipitating debris, followed by centrifugation and ion exchange chromatography. The process may include a purification step using tangential flow ultrafiltration. A scaleable process for producing pharmaceutical grade plasmid DNA, useful for gene therapy, is provided.

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