Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2005-08-23
2005-08-23
Weber, Jon (Department: 1653)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C530S350000
Reexamination Certificate
active
06933370
ABSTRACT:
The present invention provides a process for isolating and purifying highly anionic target proteins, for example, sulfated proteins. Sulfated proteins have five (5) or more, sulfations. In a preferred embodiment, the sulfated protein has six (6) sulfations, e.g., six sulfations on N-terminal tyrosine residues, as embodied in PSGL-1 (P-selectin glycoprotein ligand).
REFERENCES:
patent: 5034133 (1991-07-01), Naveh et al.
patent: 5115101 (1992-05-01), Bloom et al.
patent: 5429746 (1995-07-01), Shadle et al.
patent: 5827817 (1998-10-01), Larsen et al.
patent: 5852175 (1998-12-01), Cummings et al.
patent: 6005075 (1999-12-01), Ettlin et al.
patent: 6005081 (1999-12-01), Burton et al.
patent: 6005082 (1999-12-01), Smeds
patent: 6008036 (1999-12-01), Fanget et al.
patent: 6008328 (1999-12-01), Hsu et al.
patent: 6028191 (2000-02-01), Nardella et al.
patent: 6423831 (2002-07-01), Burton et al.
Scopes RK. Protein purification : principles and practice 2nd ed. New York : Springer-Verlag, c1987.
Li, F. et al., “Visualization of P-selectin glycoprotein ligand-1 as a highly extended molecule and mapping of protein epitopes for monoclonal antibodies,”J. Biol. Chem. Mar. 15, 1996;271(11):6342-8.
Moore, K.L. et al., “The P-selectin glycoprotein ligand from human neutrophils displays sialylated, fucosylated, O-linked poly-N-acetyllactosamine,”J. Biol. Chem. Sep. 16, 1994;269(37):23318-27.
Coffman Jonathan L.
Foster William Barry
Germain Bonnie J.
Robinson Jeffrey J.
Sun Shujun
Genetics Institute LLC
Snedden Sheridan K
Weber Jon
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