Methods for purifying highly anionic proteins

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C530S350000

Reexamination Certificate

active

06933370

ABSTRACT:
The present invention provides a process for isolating and purifying highly anionic target proteins, for example, sulfated proteins. Sulfated proteins have five (5) or more, sulfations. In a preferred embodiment, the sulfated protein has six (6) sulfations, e.g., six sulfations on N-terminal tyrosine residues, as embodied in PSGL-1 (P-selectin glycoprotein ligand).

REFERENCES:
patent: 5034133 (1991-07-01), Naveh et al.
patent: 5115101 (1992-05-01), Bloom et al.
patent: 5429746 (1995-07-01), Shadle et al.
patent: 5827817 (1998-10-01), Larsen et al.
patent: 5852175 (1998-12-01), Cummings et al.
patent: 6005075 (1999-12-01), Ettlin et al.
patent: 6005081 (1999-12-01), Burton et al.
patent: 6005082 (1999-12-01), Smeds
patent: 6008036 (1999-12-01), Fanget et al.
patent: 6008328 (1999-12-01), Hsu et al.
patent: 6028191 (2000-02-01), Nardella et al.
patent: 6423831 (2002-07-01), Burton et al.
Scopes RK. Protein purification : principles and practice 2nd ed. New York : Springer-Verlag, c1987.
Li, F. et al., “Visualization of P-selectin glycoprotein ligand-1 as a highly extended molecule and mapping of protein epitopes for monoclonal antibodies,”J. Biol. Chem. Mar. 15, 1996;271(11):6342-8.
Moore, K.L. et al., “The P-selectin glycoprotein ligand from human neutrophils displays sialylated, fucosylated, O-linked poly-N-acetyllactosamine,”J. Biol. Chem. Sep. 16, 1994;269(37):23318-27.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Methods for purifying highly anionic proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for purifying highly anionic proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for purifying highly anionic proteins will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3508603

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.