Methods for purifying authentic IGF from yeast hosts

Details Methods for purifying authentic IGF from yeast hosts Methods for purifying authentic IGF from yeast hosts

2007-03-20

2007-03-20

Gupta, Anish (Department: 1654)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

C530S303000, C530S399000, C530S408000, C530S409000, C514S012200, C435S069100, C435S069400

Reexamination Certificate

active

08477984

ABSTRACT:
Methods for purifying authentic, properly folded IGF polypeptides from yeast transformants are disclosed. The methods include a refolding step and provide for high yields of authentic IGF from a variety of yeast strains.

REFERENCES:
patent: 5231178 (1993-07-01), Holtz et al.
patent: 5288931 (1994-02-01), Chang et al.
patent: 5324639 (1994-06-01), Brierley et al.
patent: 5407810 (1995-04-01), Builder et al.
patent: 5410026 (1995-04-01), Chang et al.
patent: 5446024 (1995-08-01), Builder et al.
patent: 5451660 (1995-09-01), Builder et al.
patent: 5650496 (1997-07-01), Brierley et al.
patent: 5695958 (1997-12-01), Builder et al.
patent: 5712249 (1998-01-01), Holloran et al.
patent: 6117983 (2000-09-01), Cowgill et al.
patent: 0 567 554 (1993-11-01), None
patent: WO 92/12993 (1992-08-01), None
patent: WO 93/11240 (1993-06-01), None
patent: WO 93/19084 (1993-09-01), None
patent: WO 95/06059 (1995-03-01), None
patent: WO 95/06064 (1995-03-01), None
patent: WO 95/16701 (1995-06-01), None
patent: WO 95/16777 (1995-06-01), None
patent: WO 96/07744 (1996-03-01), None
K. Axelsson et al., “Disulfide arragnement of human insulin-like growth factor I derived from yeast and plasma”Eur. J. Biochem.(1992) 206:987-994.
J.Y. Chang et al., “Single-Step Solubilization and Folding of IGF-1 Aggregates fromEscherichia coli” Protein Folding: in vivo and in vitro(American Chemical Society, 1993) pp. 178-188.
S. Elliott et al., “Yeast-Derived Recombinant Human Insulin-Like Growth Factor I: Production, Purification, and Structural Characterization”J. Protein Chem.(1990) 9:95-104.
G. Forsberg et al., “Separation and characterization of modified variants of recombinant human insulin-like growth factor I derived from a fusion protein secreted fromEscherichia coli” Biochem. J.(1990) 271:357-363.
R.A. Hart et al. “Effect of environment on insulin-like growth factor I refolding selectivity”Biotechnol. Appl. Biochem.(1994) 20:217-234.
K.R. Hejnaes et al., “Development of an optimized refolding process for recombinant Ala-Glu-IGF-I”Prot. Eng.(1992) 5:797-806.
S. Hober et al., “Disulfide Exchange Folding of Insulin-like Growth Factor I”Biochem.(1992) 31:1749-1756.
H. Meng et al., “Reduction Studies on Bacterial Recombinant Somatomedin C/Insulin-like Growth Factor”J. Chromatog.(1988) 443:183-192.
J.A. Miller et al., “Oxidative Refolding of Insulin-like Growth Factor I Yields Two Products of Similar Thermodynamic Stability: A Bifurcating Protien-Folding Pathway”Biochemistry(1993) 32:5203-5213.
L.O. Narhi et al., “role of Native Disulfide Bonds in the Structure and Activity of Insulin-like Growth Factor I: Genetic Models of Protein-Folding Intermediates”Biochemistry(1993) 32:5214-5221.
M. Niwa et al., “Chemical Synthesis, cloning, and Expression of Genes for Human Somatomedin C (Insulin-like Growth Factor I) and59Val-Somatomedin C”Ann. NY Acad. Sci.(1986) 469:31-52.
E. Samuelsson et al. “Enhancedin VitroRefolding of Insulin-like Growth Factor I using a Solubilizing Fusion Partner”Biochemistry(1994) 33:4207-4211.

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