Methods for purifying and synthesizing heat shock protein comple

Details Methods for purifying and synthesizing heat shock protein comple Methods for purifying and synthesizing heat shock protein comple

1996-09-20

1998-05-05

Hutzell, Paula K.

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Treating animal or plant material or micro-organism

435803, 530413, 530350, 530412, 210656, 210660, 514 46, 536 276, C07K 14435, C07K 1700, A61L 3817, G01N 3002

Patent

active

057473323

ABSTRACT:
A method for purifying heat shock protein complexes is provided which comprises the steps of adding a solution containing heat shock protein complexes, in which heat shock proteins are associated with peptides, polypeptides, denatured proteins or antigens, to a column containing an ADP matrix to bind the heat shock proteins complexes to the ADP matrix and adding a buffer containing ADP to the column to remove the heat shock protein complexes in an elution product. Additionally a method for synthesizing heat shock protein complexes and purifying the complexes so produced is provided which comprises the steps of adding heat shock proteins to an ADP matrix column to bind them to the matrix, adding a solution of peptides, polypeptides, denatured proteins or antigens to the column to bind them to the heat shock proteins as heat shock protein complexes and adding a buffer containing ADP to the column to remove the complexes in an elution product.

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