Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-02-20
1994-05-03
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 792, 435971, 436513, 436518, 436536, 436538, 530413, 530825, 530863, G01N 3353, C07K 318
Patent
active
053087534
ABSTRACT:
Methods for purifying and detecting IgM antibodies employ binding substances which are Borellia burgdorferi cells, or cellular or extracellular components obtained or derived therefrom and which bind to this class of antibodies. The binding substances may be attached to a solid substrate and then the substrate contacted with a solution containing IgM antibodies under conditions such that the antibodies bind to the binding substance on the substrate. The substrate is then contacted with a solution that releases the IgM antibodies from the substrate and the antibodies are recovered or detected. Applications of these methods include, for example, assays for diagnosing diseases which elicit primary and/or secondary IgM antibody-mediated immunity.
REFERENCES:
patent: 5077391 (1991-12-01), Raison et al.
patent: 5187065 (1993-02-01), Schutzer
Craft et al., "Antigens of Borvelia burgdorferi recognized during Lyme Disease", J. Clin. Invest., 78:934-939 (Oct. 1986).
Benach et al., "A Murine IgM Monoclonal Antibody Binds an Antigenic Determinant in Outer Surface Protein A.sub.r An Immunodominant Basic Protein of the Lyme Disease Spirochete", J. Immunol., 140:265-272 (Jan. 1988).
Dorward et al., "Immune Capture and Detection of Borrelia burgdorferi Antigens in Urine, Blood or Tissues from Infected Ticks, Mice, Dogs and Humans", J. Clin. Microbiol., 29(6):1162-1170 (Jun. 1991).
Hanson et al., "Measurement of Antibodies to Borrelia burgdorferi Flagellum Improves Serodiagnosis in Lyme Disease", J. Clin. Microbiol., 26(2):338-346 (Feb. 1988).
Davis Gary
Dorward David W.
Garon Claude F.
Huguenel Edward D.
Bidwell Carol E.
Kepplinger Esther L.
The Government of the United States of America as represented by
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