Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or... – Recovery or purification
Reexamination Certificate
2007-04-24
2010-12-14
Lucas, Zachariah (Department: 1648)
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
Recovery or purification
C435S235100, C435S236000, C435S261000, C435S005000, C435S173900, C435S320100, C424S184100, C424S204100, C424S233100, C424S093600, C536S023100
Reexamination Certificate
active
07851196
ABSTRACT:
The present invention provides methods of purifying encapsidated virus, e.g., viral particles comprising viral nucleic acid, from compositions comprising encapsidated viral nucleic acid and viral particles that lack viral nucleic acid; methods for reducing the particle:genome ratio in a preparation of encapsidated viral nucleic acid; and methods for selectively inactivating viral particles that lack viral nucleic acid in a liquid composition comprising encapsidated viral nucleic acid and the viral particles that lack viral nucleic acid. The methods generally involve subjecting the composition to hydrostatic pressure such that the viral particles lacking viral nucleic acid are selectively inactivated.
REFERENCES:
patent: 7625570 (2009-12-01), Schaffer et al.
Leonard et al., Enhanced Preparation of Adeno-Associated Viral Vectors by Using High Hydrostatic Pressure to Selectively Inactivate Helper Adenovirus, 2007, Biotechnology and Bioengineering, vol. 97, No. 5, pp. 1170-1179.
Pontes et al., “Pressure inactivation of animal viruses: potential biotechnological applications”,High Pressure Research in the Biosciences and Biotechnology, H. K, Editor. 1997, p. 91-94.
Wilkinson et al., “Resistance of poliovirus to inactivation by high hydrostatic pressures”,Innovative Food Science and Emerging Technologies. 2001. 2:95-98.
Leonard Joshua N.
Schaffer David V.
Blumel Benjamin P
Borden Paula A.
Bozicevic Field & Francis LLP
Lucas Zachariah
The Regents of the University of California
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