Methods for purification of single-chain and double-chain tissue

Details Methods for purification of single-chain and double-chain tissue Methods for purification of single-chain and double-chain tissue

1987-04-29

1990-02-06

Huleatt, Jayme A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435226, 435814, 435815, C12N 948, C12N 964

Patent

active

048988251

ABSTRACT:
A mixture containing a single-chain tissue plasminogen activator (tPA) and/or double-chain tPA is brought into close contact with a column carrying an immobilized Erythrina trypsin inhibitor as an affinity agent. Adsorbed protiens are eluted with eluents having different pHs with or without arginine or benzamidine, so that single-chain tPA is obtained in the eluent with a pH at least 4.5 and double-chain tPA is obtained in the eluent with a pH lower than 4.5.

REFERENCES:
patent: 4766075 (1988-08-01), Goeddel et al.

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