Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Reexamination Certificate
2004-03-15
2008-09-16
Gitomer, Ralph (Department: 1657)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
C435S173400
Reexamination Certificate
active
07425426
ABSTRACT:
The invention provides methods for purifying one or more cells based on the level of one or more products secreted by the cells. In one embodiment, the method involves (a) contacting a plurality of cells immobilized in proximity to a capture matrix, the capture matrix capable of localizing a product secreted by one or more of the cells, with an agent that selectively binds to the product, the agent capable of generating a signal detectable as a property of light; (b) illuminating a population of the cells, the population contained in a frame; (c) detecting two or more properties of light directed from the frame, wherein a first property of light identifies substantially all cells of the population, and the second property of light identifies product localized to the capture matrix; (d) locating (i) substantially all cells of the population with reference to the detected first property of light, and (ii) one or more selected cells with reference to the detected second property of light, and (e) irradiating the non-selected cells, wherein each non-selected cell receives a substantially lethal dose of radiation, whereby one or more selected cells having a desired product secretion profile are purified.
REFERENCES:
patent: 3674926 (1972-07-01), Dewey et al.
patent: 4000417 (1976-12-01), Adkisson et al.
patent: 4165149 (1979-08-01), Suzki et al.
patent: 4284897 (1981-08-01), Sawamura et al.
patent: 4395397 (1983-07-01), Shapiro
patent: 4532402 (1985-07-01), Overbeck
patent: 4624915 (1986-11-01), Schindler et al.
patent: 4629687 (1986-12-01), Schindler et al.
patent: 4803992 (1989-02-01), Lemelson
patent: 4950592 (1990-08-01), Daiss
patent: 4998284 (1991-03-01), Bacus et al.
patent: 5013660 (1991-05-01), Kasuya et al.
patent: 5031099 (1991-07-01), Kettler
patent: 5035693 (1991-07-01), Kratzer et al.
patent: 5053693 (1991-10-01), Bohnert et al.
patent: 5089384 (1992-02-01), Hale
patent: 5093866 (1992-03-01), Douglas-Hamilton et al.
patent: 5103660 (1992-04-01), Johnson
patent: 5109276 (1992-04-01), Nudelman et al.
patent: 5158889 (1992-10-01), Hirako et al.
patent: 5188633 (1993-02-01), Kratzer et al.
patent: 5202230 (1993-04-01), Kamentsky
patent: 5235522 (1993-08-01), Bacus
patent: 5257182 (1993-10-01), Luck et al.
patent: 5272081 (1993-12-01), Weinreb et al.
patent: 5287272 (1994-02-01), Rutenberg et al.
patent: 5296963 (1994-03-01), Murakami et al.
patent: 5298963 (1994-03-01), Moriya et al.
patent: 5381224 (1995-01-01), Dixon et al.
patent: 5422720 (1995-06-01), Berndt
patent: 5428690 (1995-06-01), Bacus et al.
patent: 5432865 (1995-07-01), Kasdan et al.
patent: 5523543 (1996-06-01), Hunter, Jr. et al.
patent: 5590168 (1996-12-01), Iketaki
patent: 5646411 (1997-07-01), Kain et al.
patent: 5672880 (1997-09-01), Kain
patent: 5690846 (1997-11-01), Okada et al.
patent: 5719391 (1998-02-01), Kain
patent: 5732150 (1998-03-01), Zhou et al.
patent: 5784162 (1998-07-01), Cabib et al.
patent: 5785703 (1998-07-01), Goodman et al.
patent: 5790710 (1998-08-01), Price et al.
patent: 5795755 (1998-08-01), Lemelson
patent: 5828776 (1998-10-01), Lee et al.
patent: 5874266 (1999-02-01), Palsson
patent: 5890846 (1999-04-01), Clark et al.
patent: 5932872 (1999-08-01), Price
patent: 5952651 (1999-09-01), Morito et al.
patent: 5995143 (1999-11-01), Price et al.
patent: 6005256 (1999-12-01), McGlynn et al.
patent: 6007814 (1999-12-01), Scheinberg
patent: 6040139 (2000-03-01), Bova
patent: 6099522 (2000-08-01), Knopp et al.
patent: 6122396 (2000-09-01), King et al.
patent: 6143535 (2000-11-01), Palsson
patent: 6148096 (2000-11-01), Pressman et al.
patent: 6166385 (2000-12-01), Webb et al.
patent: 6215892 (2001-04-01), Douglass et al.
patent: 6218132 (2001-04-01), Spack et al.
patent: 6275777 (2001-08-01), Shimizu
patent: 6298264 (2001-10-01), Zhong et al.
patent: 6315772 (2001-11-01), Marchitto et al.
patent: 6381224 (2002-04-01), Lane et al.
patent: 6424863 (2002-07-01), Flock
patent: 6509166 (2003-01-01), Edberg
patent: 6514722 (2003-02-01), Palsson et al.
patent: 6534308 (2003-03-01), Palsson et al.
patent: 6642018 (2003-11-01), Koller et al.
patent: 6753161 (2004-06-01), Koller et al.
patent: 6804385 (2004-10-01), Eisfeld et al.
patent: 7092557 (2006-08-01), Eisfeld et al.
patent: 7129070 (2006-10-01), Palsson
patent: 7132289 (2006-11-01), Kobayashi et al.
patent: 2003/0219892 (2003-11-01), Palsson et al.
patent: 2004/0180437 (2004-09-01), Koller et al.
patent: 2005/0095578 (2005-05-01), Koller et al.
patent: 2005/0118652 (2005-06-01), Lee et al.
patent: 0 662 512 (1995-07-01), None
patent: 63-259465 (1987-04-01), None
patent: 2054486 (1996-02-01), None
patent: WO 89/01630 (1989-02-01), None
patent: WO 97/11156 (1997-03-01), None
patent: WO 98/42356 (1998-10-01), None
patent: WO 98/52016 (1998-11-01), None
patent: WO 01/40454 (2001-06-01), None
Bird et al., “4-Hydroperoxycyclophosphamide Purged Autologous Bone Marrow Transplantation in Non-Hodgkin's Lymphoma Patients at High Risk of Bone Marrow Involvement,”Bone Marr. Transplan., 18:309-313 (1996).
Clarke et al., “A Recombinant Bcl-XsAdenovirus Selectively Induces Apoptosis in Cancer Cells but Not in Normal Bone Marrow Cells,”Proc. Natl. Acad. Sci., 92:11024-11028 (1995).
Denk, “Two-Photon Scanning Photochemical Microscopy: Mapping Ligand-Gated Ion Channel Distributions,”Proc. Natl. Acad. Sci., 91:6629-6633 (1994).
Dooley et al., “A Novel, Inexpensive Technique for the Removal of Breast Cancer Cells from Mobilized Peripheral Blood Stem Cell Products,”Blood, 88:252a, Abstract 995 (1996).
Gazitt et al., “Purified CD34+Lin-Thy+Stem Cells Do Not Contain Clonal Myeloma Cells,”Blood, 86(1):381-389 (1995).
Grate et al., “Laser-Mediated, Site-Specific Inactivation of RNA Transcripts,”Proc. Natl. Acad. Sci., 96:6131-6136 (1999).
Gribben et al., “Immunologic Purging of Marrow Assessed by PCR Before Autologous Bone Marrow Transplantation for B-Cell Lymphoma,”New Engl. J. Med., 325(22):1525-1533 (1991).
Gulliya et al., “Elimination of Clonogenic Tumor Cells from HL-60, Daudi, and U-937 Cell Lines by Laser Photoradiation Therapy: Implications for Autologous Bone Marrow Purging,”Blood, 73(4):1059-1065 (1989).
Guo et al., “Laser-mediated Gene Transfer in Rice,”Physiol. Plantar., 93:19-24 (1995).
Hanania et al., “A Novel Automated Method of Scanning Cytometry and Laser-Induced Necrosis Applied to Tumor Cell Purging,”Blood, W.B. Sanders Company, Orlando, FL., vol. 94, No. 10, Dec. 1999, pp. 638A-639A.
Jay, “Selective Destruction of Protein Function by Chromophore-Assisted Laser Inactivation,”Proc. Natl. Acad. Sci., 85:5454-5458 (1988).
Krasieva et al., “Mechanisms of Cell Permeabilization by Laser Microirradiation,” LAMMP, Beckman Laser Institute, University of California at Irvine, Irvine, California, 8 pp., SPIE Proceedings (1999).
Mapara et al., “Monitoring of Tumor Cell Purging After Highly Efficient Immunomagnetic Selection of CD34 Cells from Leukapheresis Products in Breast Cancer Patients: Comparison of Immunocytochemical Tumor Cell Staining and Reverse Transcriptase—Polymerase Chain Reaction,”Blood, 89(1):337-344 (1997).
Mapara et al., “Combined Positive/Negative Purging and Transplantation of Peripheral Blood Progenitor Cell Autografts in Breast Cancer Patients: A Pilot Study,”Exper. Hemat., 27:169-175 (1999).
Oleinick et al., “The Photobiology of Photodynamic Therapy: Cellular Targets and Mechanisms,”Rad. Res., 150:S146-S156 (1998).
Palumbo et al., “Targeted Gene Transfer in Eucaryotic Cells by Dye-Assisted Laser Optoporation,”J. Photochem. Photobiol. B:Biol., 36:41-46 (1996).
Paulus et al., “Combined Positive/Negative Selection for Highly Effective Purging of PBPC Grafts: Towards Clinical Application in Patients with B-CLL,”Bone Marr. Transplan., 20:415-420 (1997).
Robertson et al., “Human Bone Marow Depleted of CD33-Positive Cells Mediates Delayed but Durable Reconstitution of Hematopoiesis: Clinical Tria
Eisfeld Timothy M.
Fieck Annabeth
Hanania Elie G.
Koller Manfred R.
Cyntellect, Inc.
Gitomer Ralph
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