Methods for protein binding enzyme complementation assays

Details Methods for protein binding enzyme complementation assays Methods for protein binding enzyme complementation assays

1995-06-01

1997-07-01

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 79, 435 18, 435975, 436501, 436537, 436544, G01N 33535

Patent

active

056437340

ABSTRACT:
This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques, DNA synthesis or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of .beta.-galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.

REFERENCES:
patent: 4708929 (1987-11-01), Henderson

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