Methods for protein binding enzyme complementation

Details Methods for protein binding enzyme complementation Methods for protein binding enzyme complementation

1994-06-30

1997-02-18

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 76, 435 78, 435 79, 435 18, 4351723, 435207, 436501, 436512, 436537, 436544, 530309, 530324, 530807, 930240, 935 47, G01N 33535, G01N 33542, G01N 33569

Patent

active

056040911

ABSTRACT:
This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques, DNA synthesis or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of .beta.-galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.

REFERENCES:
patent: 4378428 (1983-03-01), Farina et al.
patent: 4708929 (1987-11-01), Henderson
patent: 4745055 (1988-05-01), Schenk et al.
Daniel R. Henderson et al., Clin. Chem. 32/9 1637-1641 (1986).
Rose et al. Methods in Enzymology 101: 167-182 (1983).
Ngo et al. in Immunoenzyme techniques p. 281 (1985).
Senmetto (ed) 4th (ed), Manual of Clinical Microbiology, pp. 823-832, (1985).

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