Methods for production of recombinant plasmids

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 691, 435 911, 435 914, 435183, 4352523, 43525233, 4353201, 435325, 536 231, 536 237, C12N 1500, C12N 120, C12N 500, C07H 2104

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059225831

ABSTRACT:
The invention relates to a culture system for stable and high-level production of DNA contained on recombinant plasmids, and to the bacteria and plasmids which comprise the culture system. The bacterial cell chromosome is irreversibly altered, in one embodiment, so as to produce a substance toxic to the bacterium; in a second embodiment so as to render the cells unable to synthesize and assimilate an essential metabolite, and, in a third embodiment, to be incapable of producing a required intracellular protein that does not lead to a secreted product. In every case, the recombinant plasmid includes genetic material which functionally complements the chromosomal alteration. If the DNA on the plasmid is to be used in therapeutic applications or for administration to eukaryotes, the genetic material will have no functional or structural equivalent in eukaryotic cells, and will not result in production of mRNA or a polypeptide that acts on any eukaryotic cell component. Any peptide produced is, desirably, not toxic to the bacterial cells.

REFERENCES:
patent: 4190495 (1980-02-01), Curtiss, III
patent: 4550081 (1985-10-01), Stocker
patent: 4735801 (1988-04-01), Stocker
patent: 4760022 (1988-07-01), Molin et al.
patent: 4837151 (1989-06-01), Stocker
patent: 4888170 (1989-12-01), Curtiss, III
patent: 4920048 (1990-04-01), Diderichsen
patent: 4968619 (1990-11-01), Curtiss, III
patent: 5077044 (1991-12-01), Stocker
patent: 5210035 (1993-05-01), Stocker
patent: 5294441 (1994-03-01), Curtiss, III
patent: 5387744 (1995-02-01), Curtiss, III et al.
patent: 5399346 (1995-03-01), Anderson et al.
patent: 5702916 (1997-12-01), Molin et al.
Saqib et al., The expression of Escherichia coli diaminopimelate decaboxylase in mouse 3T3 cells, Biochim. Biophys. Acta., vol. 1219, pp. 398-404, 1994.
Duncan et al., Purification and characterization of the D-alanyl-D-alanine-adding enzyme from escherichia coli, Biochemistry, vol. 29, pp. 2379-2386, 1990.
Mulligan, The basic science of gene therapy, Science, vol. 260, pp. 926-932, May 1993.
Orkin et al., Report and recommendations of the panel to assess the NIH investment in research on gene therapy, pp. 1-41, Dec. 1995.
Cichutek, K. "Nucleic Acid Immunization: A Prophylactic Gene Therapy?", Vaccine, 12:1520-1525.
Curtiss III, R, et al., "Stabilization of Recombinant Avirulent Vaccine Strains In Vivo," Res. Mircobiol. 141:797-805 (1990).
Diderichsen, B. "A Genetic System for Stabilization of Cloned Genes in Bacillus Subtilis", pp. 35-46 (1986).
Donnelly, J.J. et al., "Immunization with Polynucleotides: A Novel Approach to Vaccination" The Immunologist, 2: 20-26 (1994).
Donnenberg, M.S. et al., "Construction of an eae Deletion Mutant of Enteropathogenic Escherichia coli by Using a Positive-Selection Suicide Vector" American Society for Microbiology, 59:4310-4317 (1991).
Dwivedi, C.P. et al., "Instability of Plasmid-Harboring Strain of E. coli in Continuous Culture" Biotechnology and Bioengineering, 24:1465-1468 (1982).
Ferrari, E. et al., "Isolation of an Alanine Racemase Gene from Bacillus Subtilis and Its Use for Plasmid Maintenance in B. Subtilis" Biotechnology, 3:1003-1007 (1985).
Galan, J.E. et al., "Cloning and Characterization of the asd gene of Salmonella Typhimurium: Use in Stable Maintenance of Recombinant Plasmids in Salmonella Vaccine Strains" Gene, 94:29-35 (1990).
Gerdes, K "The Parb (HOK/SOK) Locus of Plasmid R1: A General Purpose Plasmid Stabilization System," Biotechnology, 6:1402-1405 (1988).
Gerdes, K. et al., "Unique Type of Plasmid Maintenance Function: Postsegregational Killing of Plasmid-free Cells" Proc. Natl. Acad. Sci. USA, 83:3116-3120 (1986).
Hodgson, C.P. "The Vector Void in Gene Therapy" Biotechnology, 13:222-225 (1995).
Imanaka, T. et al., "Phenotypic Stability of trp Operon Recombinant Plasmids in Escherichia coli" J. Gen. Microbiol, 118:253-261 (1980).
Jensen, R. B. et al., "Programmed Cell Death in Bacteria: Proteic Plasmid Stabilization Systems", Mol. Microbiology, 17:205-210 (1995).
Khan, M. et al., "Plasmid Cloning Vehicles Derived from Plasmids ColE1, F, R6K, and RK2" Methods in Enzymology, 68:268-280 (1979).
Lugtenberg, E.J. J. et al., "Temperature-Sensitive Mutants of Escherichia coli K-12 with Low Activities of the L-Alanine Adding enzyme and the D-Alanyl-D-Alanine Adding Enzyme" J. Bacteriology, 110:35-40 (1972).
Miller, J. "A Short Course in Bacterial Genetics" CSH Laboratory Press (1992).
Nakayama, K. et al., "Construction of an ASD+ Expression-Cloning Vector: Stable Maintenance and High Level Expression of Cloned Genes in a Salmonella Vaccine Strain" Biotechnology, 6:693-697 (1988).
Nilsson, J. et al., "Stabilization of Escherichia coli Trytophan-Production Vectors in Continuous Cultures: A Comparison of Three Different Systems" Biotechnology, 4:901-903 (1986).
Park, J.T. "MicroReview: Why Does Escherichia coli Recycle its Cell Wall Peptides?", Molecular Microbiology, 17:421-426 (1995).
Porter, R.D. et al., "Use of the Escherichia coli SSB Gene to Prevent Bioreactor Takeover by Plasmidless Cells" Biotechnology, 8:47-51 (1990).
Rasmussen, P.B. et al., "Genetic Analysis of the parB+ Locus of Plasmid R1" Mol. Gen. Genet., 209:22-128 (1987).
Robertson, J.S. "Safety Considerations for Nucleic Acid Vaccines" Vaccine, 12:1526-1528 (1994).
Sambrook et al., "Molecular Cloning" A Laboratory Manual.
Smith, H. "Regulatory Considerations for Nucleic Acid Vaccines" Vaccine, 12:1515-1519 (1994).
Steffes, C. et al., "The lysP Gene Encodes the Lysine-Specific Permease" Journal of Bacteriology 174:3242-3249 (1992).
Stragier, P. et al., "Regulation of Diaminopimelate Decarboxylase Synthesis in Escherichia coli", J. Mol. Biol., 168:307-320 (1983).
Ulmer, J. B. et al., "Polynucleotide Vaccines" Curr. Opin. Invest. Drugs, 2:983-989 (1993).

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