Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-06-02
1997-12-16
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 712, 43525233, C12P 2106, C12N 120
Patent
active
056984162
ABSTRACT:
Production of proteins in bacteria containing DNA sequences encoding proteins under the control of a temperature-regulated promotor is improved by growing the organisms at temperatures of less than 35.degree. C. until the late logarithmic phase. Thereafter the temperature may be raised to 36.degree. C. to 39.degree. C. Antigens produced by the method of the invention may be used as vaccines, as means for measuring efficacy of vaccines, as probes to detect antigens from clinical samples and for biochemical characterization of antigens.
REFERENCES:
Bergey's Manual of Sytematic Bacteriology, vol. 1. Krieg et al., eds.Wills & Wilkins: Baltimore, Maryland, pp. 414-417, 1984.
Qoronfleh et al. Journal of Bacteriology, 174(24):7902-7909, 1992.
Cowing et al. Proceedings of the National Academy of Sciences USA, 82:2679-2683, 1985.
Grewal et al. Journal of Clinical Microbiology, 32(5):1295-1301, 1994.
Maniatis et al. Molecular Cloning: A Laboratory Manual, pp.61-63, 68-73, and 88-92, 1982.
Sambrook et al. Molecular Cloning: A Laboratory Manual, Second Edition, pp. 18.30-18.41, 1989.
Bell Brian A.
Cassels Frederick J.
Wolf Marcia K.
Moran John Francis
Robinson Douglas W.
The United States of America as represented by the Secretary of
Wai Thanda
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