Methods for producing α-substituted carboxylic acids...

Details Methods for producing α-substituted carboxylic acids... Methods for producing α-substituted carboxylic acids...

2007-11-27

2007-11-27

Kam, Chih-Min (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

C435S195000, C435S108000, C435S116000, C435S146000, C536S023200

Reexamination Certificate

active

09751299

ABSTRACT:
In accordance with the present invention there are provided methods for producing enantiomerically pure α-substituted carboxylic acids, such as, for example, α-amino acids and α-hydroxy acids, said method comprising combining an aldehyde or ketone with a cyanide and ammonia or an anmionium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity which stereoselectively hydrolyzes the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the enantiomerically pure α-substituted carboxylic acid, such as, for example, α-amino acids and α-hydroxy acids.

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