Methods for producing transgenic plants with enhanced...

Details Methods for producing transgenic plants with enhanced... Methods for producing transgenic plants with enhanced...

2007-02-06

2007-02-06

Ibrahim, Medina A. (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

C800S298000, C435S320100, C435S468000, C435S430100

Reexamination Certificate

active

10474290

ABSTRACT:
The present invention relates to a method of producing transformants with enhanced resistance and decreased uptake of heavy metals, and a plant transformed with a P type ATPase ZntA gene that pumps out heavy metals from the cells. The transformants show better growth than wild type in environment contaminated with heavy metals and have lower heavy metal contents than wild type plants. Therefore, this method of transforming plants with ZntA or biologically active ZntA—like heavy metal pumping ATPases can be useful for developing plants for phytoremediation and also for a safe crop that has resistance to heavy metals and low heavy metal contents.

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Rensing et al., “The zntA gene ofEscherichia coliencodes a Zn(II)-translocating P-type ATPase”, Proc. Natl. Acad. Sci. USA, Dec. 1997; 94:14326-14331.
Lee et al., “Functional Expression of a Bacterial Heavy Metal Transporter in Arabidopsis Enhances Resistance to and Decrease Uptake of Heavy Metals”, Plant Physiology, Oct. 2003; 133:589-596.
Sharma et al., “The ATP Hydrolytic Activity of Purified ZntA, a Pb(II)/Cd(II)/Zn(II)-translocating ATPase fromEscherichia coli”, The Journal of Biological Chemistry (2000), 275(6):3873-3878.

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