Methods for producing thrombin

Details Methods for producing thrombin Methods for producing thrombin

1992-12-30

1995-12-19

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 691, 435 696, 4352402, 4353201, 43525411, 4352542, 43525421, 424 9464, 530350, 530380, 530381, 530382, 536 221, 536 231, 536 232, 536 235, C12N 974, C12N 120, C12N 114, C12P 2106

Patent

active

054767776

ABSTRACT:
Methods are disclosed for producing thrombin. The protein is produced from host cells transformed or transfected with DNA construct(s) containing information necessary to direct the expression of thrombin precursors. The DNA constructs generally include the following operably linked elements: a transcriptional promoter, DNA sequence encoding a gla-domainless prothrombin, and a transcriptional terminator. Thrombin precursors produced from transformed or transfected host cells are activated either in vivo or in vitro.

REFERENCES:
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Downing et al., "Human Prothrombin Activation", J. Biol. Chem. 250: 8897-8906, (Dec. 10, 1975).
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Malhotra et al., "The Kinetics of Activation of Normal and .lambda.-Carboxyglutamic Acid-deficient Prothrombins" J. Biol. Chem. 260: 279-287, (Jan. 10, 1983).
Blanchard et al., "Immunoassays of Human Prothrombin Species which correlate with Funcitonal Coagulant Activities", J. Lab. Clin. Med. 101: 242-255, (Feb., 1983).
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Glover, "Expression of Cloned Genes in Animal Cells", Gene Cloning, Ed. Brammar, Chapman and Hall, N.Y. pp. 179-219 (1984).
Foster et al., "Propeptide of Human Protein C is Necessary for .lambda.-Carboxylation", Biochemistry 26: 7003-7011, (1987).
Degen and Davie, "Nucleotide Sequence of the Gene for Human Prothrombin", Biochemistry 26: 6165-6177, (1987).
Krishnaswamy et al., "Activation of Human Prothrombin by Human Prothrombinase", J. Biol. Chem. 262:3291-3299 (1987).
Rosing et al., "Calcium-Independent Activation of Prothromnin on Membranes with Positively Charged Lipids" Biochem. 27:9048-9055 (1988).
Teng et al. "Effects of Venom Proteases on Peptide Chromogenic Substrates and Bovine Prothrombin", Taxicon. 27: 161-167, (1989).

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