Methods for producing thrombin

Details Methods for producing thrombin Methods for producing thrombin

1995-06-05

1996-03-26

Patterson, Jr., Charles L.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

424 9464, 435 691, 435 696, 435214, 4352402, 4353201, 530350, 530380, 530381, 530382, 514 2, 536 221, 536 231, 536 232, 536 235, A61K 3800, A61K 3846, C12N 974, C12P 2106

Patent

active

055020348

ABSTRACT:
Methods are disclosed for producing thrombin. The protein is produced from host cells transformed or transfected with DNA construct(s) containing information necessary to direct the expression of thrombin precursors. The DNA constructs generally include the following operably linked elements: a transcriptional promoter, DNA sequence encoding a gla-domainless prothrombin, and a transcriptional terminator. Thrombin precursors produced from transformed or transfected host cells are activated either in vivo or in vitro.

REFERENCES:
Heldebrant et al., "The Activation of Prothrombin", J. Biol. Chem. 248: 7149-7163, (Oct. 25, 1973).
Downing et al., "Human Prothrombin Activation", J. Biol. Chem. 250: 8897-8906, (Dec. 10, 1975).
Jackson and Nemerson, "Blood Coagulation", Ann. Rev. Biochem. 49: 765-811, (1980).
Malhotra et al., "The Kinetics of Activation of Normal and .lambda.-Carboxylglutamic Acid-deficient Prothrombins", J. Biol. Chem. 260: 279-287, (Jan. 10, 1983).
Blanchard et al., "Immunoassays of Human Prothrombin Species which correlate with Functional Coagulant Activities", J. Lab. Clin. Med. 101: 242-255, (Feb., 1983).
Degen et al., "Characterization of the Complementary Deoxyribonucleic Acid and Gene Coding for Human Prothrombin", Biochemistry 22: 2087-2097, (1983).
Glover, "Expression of Cloned Genes in Animal Cells", Gene Cloning Ed. Brammar, Chapman and Hall, N.Y. pp. 179-219 (1984).
Foster et al., "Propeptide of Human Protein C is Necessary for .lambda.-Carboxylation", Biochemistry 26: 7003-7011, (1987).
Degen and Davie, "Nucleotide Sequence of the Gene for Human Prothrombin", Biochemistry 26: 6165-6177, (1987).
Krishnaswamy et al., "Activation of Human Prothrombin by Human Prothrombinase", J. Biol. Chem. 262: 3291-3299, (Mar., 1987).
Rosing et al., "Calcium-Independent Activation of Prothrombin on Membranes with Positively Charged Lipids", Biochem. 27:9048-9055 (1988).
Teng et al., "Effects of Venom Proteases on Peptide Chromogenic Substrates and Bovine Prothrombin", Taxicon. 27: 161-167, (1989).
Jorgensen et al. "Expression of Completely .lambda.-Carboxylated . . . " Journal of Biological Chemistry 262: 6729-6734 (1987).
Mann "Prothrombin and Thrombin" in Hemostasis and Thrombosis: Basic Principles and Clinical Practice, Robert Colman et al. ed. Lippincott Co., Philadelphia pp. 184-186 (1994).

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