Methods for producing thrombin

Details Methods for producing thrombin Methods for producing thrombin

1995-06-05

1996-06-18

Patterson, Jr., Charles L.

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

435 691, 435 696, 435214, 4352402, 4353201, 514 2, 514 12, 530350, 530380, 530381, 530382, 536 221, 536 231, 536 232, 536 235, A61K 3846, A61K 3800, C12N 974, C12P 2106

Patent

active

055276920

ABSTRACT:
Methods are disclosed for producing thrombin. The protein is produced from host cells transformed or transfected with DNA construct(s) containing information necessary to direct the expression of thrombin precursors. The DNA constructs generally include the following operably linked elements: a transcriptional promoter, DNA sequence encoding a gla-domainless prothrombin, and a transcriptional terminator. Thrombin precursors produced from transformed or transfected host cells are activated either in vivo or in vitro.

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