Multicellular living organisms and unmodified parts thereof and – Method of making a transgenic nonhuman animal – Via microinjection of dna into an embryo – egg cell – or...
Patent
1997-07-21
1999-07-13
Crouch, Deborah
Multicellular living organisms and unmodified parts thereof and
Method of making a transgenic nonhuman animal
Via microinjection of dna into an embryo, egg cell, or...
800 22, 800 18, 435455, 435463, 435 691, 4353201, 435325, C12N 500, C12N 1500, C12N 1509, C12N 1563
Patent
active
059229276
ABSTRACT:
Transgenic mice carrying two transgenes, the first coding for a transactivator fusion protein comprising a tet repressor and a polypeptide which directly or indirectly activates in eucaryotic cells, and the second comprising a gene operably linked to a minimal promoter operably linked to at least one tet operator sequence, are disclosed. Isolated DNA molecules (e.g., targeting vectors) for integrating a polynucleotide sequence encoding a transactivator of the invention at a predetermined location within a second target DNA molecule by homologous recombination are also disclosed. Transgenic mice having the DNA molecules of the invention integrated at a predetermined location in a chromosome by homologous recombination are also encompassed by the invention. Methods to regulate the expression of a tet operator linked-gene of interest by administering tetracycline or a tetracycline analogue to a mouse of the invention are also disclosed. The regulatory system of the invention allows for conditional inactivation or modulation of expression of a gene of interest in a host cell or mouse.
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Bujard Hermann
Gossen Manfred
Salfeld Jochen G.
Voss Jeffrey W.
BASF - Aktiengesellschaft
Crouch Deborah
Martin Jill D.
LandOfFree
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