Methods for producing soluble membrane-spanning proteins

Details Methods for producing soluble membrane-spanning proteins Methods for producing soluble membrane-spanning proteins

2009-08-14

2010-11-09

Desai, Anand U (Department: 1656)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S320100, C435S476000, C435S069100, C435S490000

Reexamination Certificate

active

07829684

ABSTRACT:
Methods for producing membrane-spanning polypeptides in high yields, with native conformation, and/or in soluble form include solubilizing in non-ionic or zwitterionic detergents, as well as use of promoters and expression vectors for expressing high yields of membrane-spanning polypeptides in bacterial cells. Mutated promoters provide tight control of membrane-spanning polypeptides in bacterial cell hosts.

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