Methods for producing secreted polypeptides

Details Methods for producing secreted polypeptides Methods for producing secreted polypeptides

2008-07-01

2008-07-01

Vogel, Nancy (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S254110

Reexamination Certificate

active

07393664

ABSTRACT:
The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3′ end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.

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Heijne Won G. A new method for predicting signal sequence cleavage sites, Nucleic Acids Research, V. 14, No. 11, 1986. pp. 4683-4690.

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