Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1994-12-05
1999-10-05
Ketter, James
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351721, 4351723, 4352523, 43525233, 4353201, 435 9141, 935 22, 935 23, 935 24, C12P 2106, C12N 1500, C12N 1563, C12N 120
Patent
active
059622555
ABSTRACT:
Methods, recombinant host cells and kits are disclosed for the production of members of specific binding pairs (sbp), e.g. antibodies, using display on the surface of secreted replicable genetic display packages (rgdps), e.g. filamentous phage. To produce a library of great diversity recombination occurs between first and second vectors comprising nucleic acid encoding first and second polypeptide chains of sbp members respectively, thereby producing recombinant vectors each encoding both a first and a second polypeptide chain component of a sbp member. The recombination may take place in vitro or intracellularly and may be site-specific, e.g. involving use of the loxP sequence and mutants thereof. Recombination may take place after prior screening or selecting for rgdps displaying sbp members which bind complementary sbp member of interest.
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Griffiths Andrew David
Johnson Kevin Stuart
Nissim Ahuva
Smith Andrew John Hammond
Waterhouse Peter Michael
Cambridge Antibody Technology Limited
Ketter James
Medical Research Council
Wai Thanda
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