Methods for producing proteins using silkworm middle silk...

Details Methods for producing proteins using silkworm middle silk... Methods for producing proteins using silkworm middle silk...

2005-03-21

2008-12-02

Wehbe, Anne Marie (Department: 1633)

Multicellular living organisms and unmodified parts thereof and

Method of using a transgenic nonhuman animal to manufacture...

C800S022000, C800S008000

Reexamination Certificate

active

07459599

ABSTRACT:
Transgenic silkworms comprising GFP whose expression is regulated by the sericin gene promoter were produced. Observation of the silk glands of the last instar larvae of the silkworms showed fluorescence only in the middle silk glands. GFP was secreted from middle silkgland cells from around the spinning stage, indicating that GFP moved into the gland lumen. Finally, GFP was spun into cocoon filaments, and cocoons containing large amounts of GFP were produced. Thus, by using the promoter region of the sericin gene, recombinant proteins can be produced in the middle silk glands. Furthermore, the recombinant proteins produced in the middle silk glands were readily secreted into the lumen of the middle silk glands.

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