Methods for producing proteins in cultured cells

Details Methods for producing proteins in cultured cells Methods for producing proteins in cultured cells

2005-05-10

2005-05-10

Carlson, Karen Cochrane (Department: 1653)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S358000, C536S022100, C536S026300

Reexamination Certificate

active

06890736

ABSTRACT:
The invention provides a method for increasing production of a protein by cultured mammalian cells comprising adding a DNA demethylating agent, for example a cytidine analogue, to the mammalian cell culture. The cytidine analogue can be 5-aza-2′-deoxycytidine (also called decitabine) or 5-bromo-2′-deoxycytidine-5′-monophosphate, among many other possible cytidine analogues.

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