Methods for producing proteins by using cell-free protein...

Details Methods for producing proteins by using cell-free protein... Methods for producing proteins by using cell-free protein...

2005-10-04

2005-10-04

Weber, Jon (Department: 1653)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

C435S004000, C435S069100, C435S069900, C435S071100, C435S071200, C530S219000, C530S224000, C530S333000, C530S417000

Reexamination Certificate

active

06951734

ABSTRACT:
A method for producing a protein suitable for X-ray crystallographic analysis, in a cell-free protein synthesis system comprising a cell-free extract, a nucleic acid coding for said protein, and amino acids for the substrate of said protein, wherein said amino acids comprises at least one amino acid comprising a heavy atom, and wherein the introduced rate of said amino acid comprising the heavy atom into the synthesized protein is at least 80%.

REFERENCES:
patent: 1143009 (2001-10-01), None
patent: A-4-200390 (1992-07-01), None
patent: A-2000-175695 (2000-06-01), None
Sprin wt al., Science, vol. 242, pp. 1162-1164, Nov. 25, 1988.
Hendrickson. Science, vol. 254, pp. 51-58, Oct. 4, 1991.
Hendrickson. “Determination of Macromolecular Structures from Anomalous Diffraction of Synchrotron Radiation” Science 254:51-58 (1991).
Kigawa et al. “Structure Determination of Protein Folds Using the Cell-free Synthesis and NMR Spectroscopy” Experimental Medicine 18(18):60-64 (2000).
Spirin et al. “A Continuous Cell-Free Translation System Capable of Producing Polypeptides in High Yield” Science 242:1162-1164 (1988).

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