Methods for producing probes capable of distingushing variant ge

Details Methods for producing probes capable of distingushing variant ge Methods for producing probes capable of distingushing variant ge

1992-11-12

1995-07-25

Parr, Margaret

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 536 243, 536 2431, 935 78, C12P 1934

Patent

active

054361427

ABSTRACT:
Methodology is provided for developing probes for identifying sequence differences between two related DNA populations, sets of DNA fragments or collections of restriction-endonuclease-cleaved DNA. The method employs an initial stage to obtain a representation of both DNA populations, namely using the PCR to produce relatively short fragments, referred to as amplicons. Tester amplicons containing target DNA, sequences of interest, are ligated to adaptors and mixed with excess driver amplicons under melting and annealing conditions, followed by PCR amplification. The process may be repeated so as to greatly enrich the target DNA. Optionally, the target DNA may then be cloned and the DNA used as probes.

REFERENCES:
Welcher et al., Nucleic Acids Res. 14(24), 10027-10044 (1986).
Bjourson et al., Appl. Environ. Micro. 54(11), 2852-2855 (1988).
Lisitsyn et al., Science 259, 946-951 (1993).
Lamar and Palmer (1984) Y-encoded, species-specific DNA in mice: evidence that the Y chromosome exists in two polymorphic forms in inbred strains. Cell 37:171-177.
Kunkel, et al. (1985) Specific cloning of DNA fragments absent from the DNA of a male patient with an X chromosome deletion. Proc. Natl. Acad. Sci. USA 82:4778-4782.
Nussbaum, et al. (1987) Isolation of anonymous DNA sequences from within a submicroscopic X chromosomal deletion in a patient with choroideremia, deafness, and mental retardation. Proc. Natl. Acad. Sci. USA 84:6521-6525.
Wieland, et al. (1990) A method for difference cloning: gene amplification following subtractive hybridization. Proc. Natl. Acad. Sci. USA 87:2720-2724.
Straus and Ausubel (1990) Genomic subtraction for cloning DNA corresponding to deletion mutations. Proc. Natl. Acad. Sci. USA 87:1889-1893.
Wieland, et al. (1992) Isolation of DNA sequences deleted in lung cancer by genomic difference cloning. Proc. Natl. Acad. Sci. USA 89:9705-9709.

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